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KMID : 0371919890020010051
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Journal of Wonju College of Medicine
1989 Volume.2 No. 1 p.51 ~ p.59
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Effects of Actinomycin D on the Ultrastructre and the Contents of the Nucleic Acids and Protein in Mouse Liver
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Abstract
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Actinomycin D which inhibitis RNA synthesis was injected into mouse to investigate, at intervals, the effects on the ultrastructure and contents of DNA, RNA, and protein in liver.
Ultrastructural changes resulting from the injection of Actinomycin D were first observed in nucleolus. In groups injected with lower concentration, nucleolar changes were gradually reorganized in the course of time, and the changes in the higher, however, were kept for up to 96 hours after the injection, and cytoplasmic changes were also observed. Those ultrastructural alterations were characterized by the nucleolar condensation, irregular nuclear mumbrane, the dilatation of rough endoplasmic reticulum, increases of the lipid droplets and glycofen particles.
DNA content decreased in higher injected group, but it was not changed in the lower. RNA content decreased in the groups treated with Actinomycin D. Protein content decreased for up to 24 hours, showed the similar level to the controls at 48 hours, and increased more than the controls at 96 hours.
Actinomycin D induced the ultrastructural changes and also influenced on the contents of the nucleic acids and protein in mouse liver. From the results, it was suggested that these effects could be correlated with each other and were dose dependent.
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KEYWORD
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